Characterization of Excretory/ Secretory Antigen of Bunostomum Trigonocephalum in Sheep
نویسنده
چکیده
In the present study, E/S antigen from Bunostomum trigonocephalum was prepared by in vitro culture method and hyper immune serum was raised against this antigen in rabbits. On SDS – PAGE analysis, the E/S antigen revealed six polypeptides at 21.0, 29.0, 47.0, 60.0, 94.0 and 101.0 kDa. Further, three immuno-reactive polypeptides at 29.0, 47.0 and 60.0 kDa were detected in western blotting. This antigenic characterization study will be helpful in developing suitable sero diagnostic in bunostomosis in sheep.
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تاریخ انتشار 2013